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Science 2 September 1966:
Vol. 153. no. 3740, pp. 1116 - 1119
DOI: 10.1126/science.153.3740.1116
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Articles

Differentiation in vitro: Effects of Sephadex Fractions of Chick Embryo Extract

Hayden G. Coon 1 and Robert D. Cahn 2

1 Biology Department, Brandeis University, Waltham, Massachusetts
2 Zoology Department, University of Washington, Seattle 98105

Chicken embryo extract has been fractionated into high-and low-molecular-weight components on Sephadex G-25. Media supplemented with the low-molecular-weight fraction (L) support full differentiation in clones of cartilage and of pigmented retina cells from chicken embryos. Growth rates of such cultures in L-supplemented media are greater than in media without embryo extract, and plating efficiencies are higher than in media with or without whole embryo extract. The high-molecular-weight fraction (H) in low concentrations also stimulates growth and plating efficiency, but inhibits the expression of differentiation.


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Science. ISSN 0036-8075 (print), 1095-9203 (online)