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Science 26 August 1966:
Vol. 153. no. 3739, pp. 1004 - 1006
DOI: 10.1126/science.153.3739.1004
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Articles

Immunization of Normal Mouse Spleen Cell Suspensions in vitro

Robert I. Mishell 1 and Richard W. Dutton 1

1 Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California

Dissociated cells from the spleens of unimmunized mice were cultured with and without various mammalian erythrocytes. Spleen cell suspensions cultured with heterologous red cells developed levels of hemolytic plaque-forming cells only one log2 less than those seen in vivo. The reaction is specific for the in vitro immunizing erythrocytes. Antibody was demonstrated in the culture fluids.


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