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Science 5 August 1966:
Vol. 153. no. 3736, pp. 640 - 641
DOI: 10.1126/science.153.3736.640
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Articles

Serum High-Density Lipoprotein: Effect of Change in Structure on Activity of Chicken Adipose Tissue Lipase

A. Scanu 1

1 Departments of Medicine and Biochemistry, University of Chicago, Chicago, Illinois

The high-density (1.063 to 1.21 g/ml) lipoprotein in human serum was analyzed as activator for a lipoprotein lipase isolated from chicken adipose tissue. The activating capacity was lost when the lipoprotein was extracted with a mixture of ethanol and ethyl ether (3:2 by volume) at —10°C and it was restored upon incubation of the extracted protein with aqueous sols of either whole phospholipids or the lecithin fraction prepared from the high-density lipoprotein. Since phospholipid sols alone proved ineffective as substrate activators, the complex which forms upon incubation of the extracted lipoprotein with phospholipids appears to be a necessary requirement for lipoprotein lipase activity.


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