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Science 29 July 1966:
Vol. 153. no. 3735, pp. 537 - 539
DOI: 10.1126/science.153.3735.537
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Articles

Biosynthesis of Gamma Globulin: Studies in a Cell-Free System

Kurt H. Stenzel 1 and Albert L. Rubin 1

1 Rogosin Laboratories, Department of Medicine, New York Hospital-Cornell Medical Center, New York

Incorporation of C14-amino acids into high-molecular-weight material precipitable by trichloroacetic acid indicates that microsomal cell-free systems, derived from spleens of immunized rabbits, are active in protein synthesis. Protein was made soluble by ultrasonic irradiation of the cell-free incubation mixtures, and low-molecular-weight materials were removed by dialysis and gel filtration. Chromatography and radio-immunoelectrophoresis of this soluble protein fraction reveal C14-labeled protein having several characteristics of gamma globulin.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Biosynthesis of Immunoglobulin in a Cell-Free System.
B. Mach, H. Koblet, and D. Gros (1967)
Cold Spring Harb Symp Quant Biol 32, 269-275
   Abstract »    PDF »
Immunoglobulin Synthesis in a Cell-Free System.
P. Ralph, M. Becker, and A. Rich (1967)
Cold Spring Harb Symp Quant Biol 32, 277-282
   Abstract »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)