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Science 17 June 1966:
Vol. 152. no. 3729, pp. 1631 - 1634
DOI: 10.1126/science.152.3729.1631
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Articles

Staining Tissue for Light and Electron Microscopy by Bridging Metals with Multidentate Ligands

Jacob S. Hanker 1, Chandicharan Deb 1, Hannah L. Wasserkrug 1, and Arnold M. Seligman 1

1 Departments of Surgery, Sinai Hospital of Baltimore, and Johns Hopkins University School of Medicine, Baltimore, Maryland

"Osmium black," a pigment very useful for cytological staining in both light and electron microscopy, may be deposited selectively at the tissue-binding sites of other metal ions by bridging OsO4 to the tissue bound metal ion through a multidentate ligand.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Phi bodies: peroxidatic particles that produce crystalloidal cellular inclusions.
J. Hanker and D. Romanovicz (1977)
Science 197, 895-898
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Osmiophilic Polymer Generation: Catalysis by Transition Metal Compounds in Ultrastructural Cytochemistry.
J. S. Hanker, W. A. Anderson, and F. E. Bloom (1972)
Science 175, 991-993
   Abstract »    PDF »
Coordination Polymers of Osmium: The Nature of Osmium Black.
J. S. Hanker, F. Kasler, M. G. Bloom, J. S. Copeland, and A. M. Seligman (1967)
Science 156, 1737-1738
   Abstract »    PDF »


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