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Science 27 May 1966:
Vol. 152. no. 3726, pp. 1255 - 1256
DOI: 10.1126/science.152.3726.1255
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Articles

Transformation of Auxotrophic Mutants of Group H Streptococci

C. Gomez Leonard 1, Diane Corley 1, and Roger M. Cole 1

1 National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20014

Stable auxotrophic mutants of a group H streptococcus (strain Challis) were isolated on a modified Mickelson defined medium after exposure to N-methyl- Nacute-nitro-N-nitrosoguanidine. Such mutants were transformed to both prototrophy and streptomycin-resistance and hence may be used as nutritional markers in the study of streptococcal genetics.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)