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Science 22 April 1966:
Vol. 152. no. 3721, pp. 509 - 512
DOI: 10.1126/science.152.3721.509
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Articles

Isopycnic Centrifugation for the Isolation of DNA Strands Coding for Ribosomal RNA

Peter F. Davison 1

1 Department of Biology, Massachusetts Institute of Technology, Cambridge

Denatured DNA preparations from Escherichia coli were centrifuged to equilibrium in cesium chloride solutions. Hybridizing experiments with radioactively labeled ribosomal RNA showed that the DNA strands complementary to ribosomal RNA were distributed on the heavy side of the DNA band. By fractionating this band the DNA strands coding for ribosomal RNA may be enriched 5- to 20- fold.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)