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Science 1 April 1966:
Vol. 152. no. 3718, pp. 72 - 73
DOI: 10.1126/science.152.3718.72
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Articles

Analysis of Complex Vascular Systems in Plants: Optical Shuttle Method

M. H. Zimmermann 1 and P. B. Tomlinson 2

1 Cabot Foundation, Harvard University, Harvard Forest, Petersham, Massachusetts 01366
2 Fairchild Tropical Garden, Miami, Florida 33156

The "optical shuttle" is an improved method of photographing with a motion-picture camera, one by one, sequential sections mounted on separate slides. The optical output from two microscopes is combined so that the images can be focused on a single film plane. Slides are photographed alternately through the microscopes. Simultaneous viewing of two successive sections in the microscopes enables initial precise alignment of images. Complex anatomical structures can thus be analyzed with relative ease.


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Stem vascular architecture in the rattan palm Calamus (Arecaceae-Calamoideae-Calaminae).
P. B. Tomlinson, J. B. Fisher, R. E. Spangler, and R. A. Richer (2001)
Am. J. Botany 88, 797-809
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Science. ISSN 0036-8075 (print), 1095-9203 (online)