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Science 25 March 1966:
Vol. 151. no. 3717, pp. 1528 - 1530
DOI: 10.1126/science.151.3717.1528
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Articles

Autoradiography with Tritiated Methotrexate and the Cellular Distribution of Folate Reductase

Zbigniew Darzynkiewicz 1, Andrew W. Rogers 1, Eric A. Barnard 1, Dau-Hsi Wang 2, and William C. Werkheiser 2

1 Molecular Enzymology Unit, Department of Biochemical Pharmacology, State University of New York, Buffalo 14
2 Department of Experimental Therapeutics, Roswell Park Memorial institute, Buffalo 3, News York

Bound methotrexate has been revealed by an autoradiographic procedure, presumed to introduce a method for cytochemical study of folate reductase. Preferential localization is seen in kidney proximal tubules, intestinal epithelium, and nuclei of parenchymal liver cells in mice. The extremely firm binding and prolonged retention of this drug should render it suitable as an inert label for the autoradiographic study of cell migrations and lifetimes.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)