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Science 28 January 1966:
Vol. 151. no. 3709, pp. 463 - 464
DOI: 10.1126/science.151.3709.463
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Transverse Gradient Electrophoresis: Protein Homogeneity Test and Subfractionation Technique

Clyde A. Dubbs 1

1 St. John's Hospital Research Foundation, Santa Monica, California, and Center for the Health Sciences, University of California, Los Angeles

Electrophoresis of protein (including enzyme) was conducted in a gel medium across which, transverse to the direction of protein migration, a continuous pH gradient extends. Any splitting of a resultant trace for the change of protein mobility with pH suggests both protein heterogeneity and the pH conditions under which further purification and subfractionation may best be pursued.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)