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Science 21 January 1966:
Vol. 151. no. 3708, pp. 333 - 337
DOI: 10.1126/science.151.3708.333
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Articles

Microinjection of Mouse Eggs

Teh Ping Lin 1

1 Department of Anatomy, University of California School of Medicine, San Francisco

A technique has been developed for injecting known amounts of liquids into fertilized one-celled mouse eggs by use of a calibrated ocular micrometer superimposed on the terminal regions of the injection pipette. From 128 pronuclear eggs, each injected with 180 or 770 cubic microns of bovine gamma globulin at a concentration of 25 milligrams per milliliter in citrate-Locke's solution and then transferred to the oviducts of pregnant foster mothers, 18 living fetuses developed; from 74 eggs, each injected with 2730 cubic microns, 5 fetuses survived. The living fetuses that developed from the injected eggs were smaller than normal in 6 of 23 surviving experimental fetuses as contrasted to only 1 of 19 control fetuses.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)