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Science 14 January 1966:
Vol. 151. no. 3707, pp. 203 - 205
DOI: 10.1126/science.151.3707.203
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Articles

Proteins and Disulfide Groups in the Aggregation of Dissociated Cells of Sea Sponges

Gabriel J. Gasic 1 and Norbel L. Galanti 1

1 Department of Oncology, University of Chile School of Medicine, Santiago, and Department of Pathology, University of Pennsylvania School of Medicine, Philadelphia, 19104

A sponge extract that produced specific aggregation of dissociated cells was treated with various enzyme preparations to determine which enzymes would destroy its aggregating properties. The results indicate that proteins play a key role in the aggregating effect of the extract on dissociated, glutaraldehyde-fixed sponge cells. Further studies confirm the necessity of calcium for the aggregation and indicate the necessity of intact disulfide groups.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Molluscan Cells: Dissociation and Reaggregation.
V. K. Farris (1968)
Science 160, 1245-1246
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Science. ISSN 0036-8075 (print), 1095-9203 (online)