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Science 29 October 1965:
Vol. 150. no. 3696, pp. 618 - 619
DOI: 10.1126/science.150.3696.618
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Articles

Sperm Capacitation by Uterine Fluid or Beta-Amylase in vitro

Kenneth T. Kirton 1 and Harold D. Hafs 1

1 Animal Reproduction Laboratory, Dairy Department, Michigan State University, East Lansing

Rabbit sperm developed the capacity to fertilize ova when incubated in utero or in vitro in fluids from estrous uteri. Incubation of sperm with beta-amylase in phosphate-buffered Locke's solution also resulted in capacitation. The data suggest that sperm capacitation involves enzymatic alteration of carbo-hydrate-containing seminal macromolecules which coat sperm and inhibit fertilization.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Fertilization of Rabbit Ova in vitro by Sperm with Adsorbed Sendai Virus.
R. J. Ericsson, D. A. Buthala, and J. F. Norland (1971)
Science 173, 54-55
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Science. ISSN 0036-8075 (print), 1095-9203 (online)