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Science 8 October 1965:
Vol. 150. no. 3693, pp. 217 - 221
DOI: 10.1126/science.150.3693.217
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Articles

Messenger RNA in Early Sea-Urchin Embryos: Size Classes

Martin Nemer 1 and Anthony A. Infante 1

1 Institute for Cancer Research, Fox Chase, Philadelphia 11, Pennsylvania

Rapidly labeled RNA from four-cell embryos and blastulae of sea urchins was analyzed by sedimentation and for ability to form DNA-RNA hybrids. The RNA was derived from polyribosomes and from the "gel interphase," an extraction compartment resulting from treatment of whole embryos with phenol and known to be enriched with nuclei. The RNA from both sources displayed a high degree of structural complementarity to DNA. This DNA-like RNA of the polyribosomes sedimented in discrete classes, rather than in the sedimentation continuum demonstrable for the labeled RNA of the gel interphase. Thus messenger RNA appears to emerge in the cytoplasm in discrete size classes.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Repeated Sequences in DNA.
R. J. Britten and D. E. Kohne (1968)
Science 161, 529-540
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Cilia Regeneration in the Sea Urchin Embryo: Evidence for a Pool of Ciliary Proteins.
W. Auclair and B. W. Siegel (1966)
Science 154, 913-915
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Messenger RNA in Early Sea-Urchin Embryos: Cytoplasmic Particles.
A. S. Spirin and M. Nemer (1965)
Science 150, 214-217
   Abstract »    PDF »


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