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Science 23 July 1965:
Vol. 149. no. 3682, pp. 417 - 422
DOI: 10.1126/science.149.3682.417
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Articles

Suppression in vitro: Identification of a Serine-sRNA as a "Nonsense" Suppressor

Mario R. Capecchi 1 and Gary N. Gussin 1

1 Biological Laboratories, Harvard University, Cambridge, Massachusetts

In a cell-free system, with RNA from a suppressible mutant of bacteriophage R17 as messenger,. no functional coat protein of the bacteriophage is synthesized unless serine-accepting soluble RNA from the suppressor strain, Escherichia coli S26RIE, is present.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Global Transcriptional Effects of a Suppressor tRNA and the Inactivation of the Regulator frmR.
C. D. Herring and F. R. Blattner (2004)
J. Bacteriol. 186, 6714-6720
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Ribosomal Binding Site of Release Factors RF1 and RF2. A NEW TRANSLATIONAL TERMINATION ASSAY IN VITRO.
G. Grentzmann and P. J. Kelly (1997)
J. Biol. Chem. 272, 12300-12304
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Self-Assembly of Qbeta and MS2 Phage Particles: Possible Function of Initiation Complexes.
P. P. Hung, C. M. Ling, and L. R. Overby (1969)
Science 166, 1638-1640
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Approaches to the Genetics of Escherichia coli Ribosomes.
D. Apirion, S. L. Phillips, and D. Schlessinger (1969)
Cold Spring Harb Symp Quant Biol 34, 117-128
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Genetically Altered tRNAGly Subspecies in E. coli.
J. Carbon, C. Squires, and C. W. Hill (1969)
Cold Spring Harb Symp Quant Biol 34, 505-512
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Sense and Nonsense in the Genetic Code: Three exceptional triplets can serve as both chain-terminating signals and amino acid codons.
A. Garen (1968)
Science 160, 149-159
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Azure Mutants: A Type of Host-Dependent Mutant of the Bacteriophage f2.
K. Horiuchi and N. D. Zinder (1967)
Science 156, 1618-1623
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Formylmethionyl-tRNA Dependence of Amino Acid Incorporation in Extracts of Trimethoprim-Treated Escherichia coli.
J. Eisensatadt and P. Lengyel (1966)
Science 154, 524-527
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Formylmethionine Codon AUG as an Initiator of Polypeptide Synthesis.
R. E. Thach, K. F. Dewey, J. C. Brown, and P. Doty (1966)
Science 153, 416-418
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Polynucleotide Synthesis and the Genetic Code.
H. G. Khorana, H. Buuchi, H. Ghosh, N. Gupta, T. M. Jacob, H. Kossel, R. Morgan, S. A. Narang, E. Ohtsuka, and R. D. Wells (1966)
Cold Spring Harb Symp Quant Biol 31, 39-49
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sRNA Specificity for Codon Recognition as Studied by the Ribosomal Binding Technique.
D. Soll, J. Cherayil, D. S. Jones, R. D. Faulkner, A. Hampel, R. M. Bock, and H. G. Khorana (1966)
Cold Spring Harb Symp Quant Biol 31, 51-61
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Translation of Synthetic Messenger RNA.
R. E. Thach, T. A. Sundararajan, K. F. Dewey, J. C. Brown, and P. Doty (1966)
Cold Spring Harb Symp Quant Biol 31, 85-97
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Characteristics of the Genetic Code in vivo.
M. G. Weigert, E. Gallucci, E. Lanka, and A. Garen (1966)
Cold Spring Harb Symp Quant Biol 31, 145-150
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Nonsense Codons.
A. O. W. Stretton, S. Kaplan, and S. Brenner (1966)
Cold Spring Harb Symp Quant Biol 31, 173-179
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Translation of the Structural Genes of the E. coli Pyruvate Dehydrogenase Complex.
U. Henning, G. Dennert, R. Hertel, and W. S. Shipp (1966)
Cold Spring Harb Symp Quant Biol 31, 227-234
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Polarity in the Tryptophan Operon of E. coli.
F. Imamoto, J. Ito, and C. Yanofsky (1966)
Cold Spring Harb Symp Quant Biol 31, 235-249
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Punctuation in the Genetic Code.
N. D. Zinder, D. L. Engelhardt, and R. E. Webster (1966)
Cold Spring Harb Symp Quant Biol 31, 251-256
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Protein Synthesis Directed by RNA Phage Messengers.
G. N. Gussin, M. R. Capecchi, J. M. Adams, J. E. Argetsinger, J. Tooze, K. Weber, and J. D. Watson (1966)
Cold Spring Harb Symp Quant Biol 31, 257-271
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Degenerate Transfer RNAs from Brewer's Yeast.
P. L. Bergquist (1966)
Cold Spring Harb Symp Quant Biol 31, 435-447
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Missense Suppression Due to a Genetically Altered tRNA.
J. Carbon, P. Berg, and C. Yanofsky (1966)
Cold Spring Harb Symp Quant Biol 31, 487-497
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Mutagenesis of Super-Suppressors in Yeast.
G. E. Magni and P. P. Puglisi (1966)
Cold Spring Harb Symp Quant Biol 31, 699-704
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