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Science 16 July 1965:
Vol. 149. no. 3681, pp. 308 - 310
DOI: 10.1126/science.149.3681.308
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Articles

Thymine Photoproducts but not Thymine Dimers Found in Ultraviolet-Irradiated Bacterial Spores

J. E. Donnellan Jr. 1 and R. B. Setlow 1

1 Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee

Bacillus megateriunm spores labeled with tritiated thymidine were irradiated with monochromatic ultraviolet light, and the DNA of the spores was analvzed for thymine-containing, products. No thymine dimers were observed, but three other thymine photoproducts were found. The unknown products of radiation were produced in vitro by irradiation of DNA that had been dried in the, presence of various salts.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
DNA Repair and Free Radicals, New Insights into the Mechanism of Spore Photoproduct Lyase Revealed by Single Amino Acid Substitution.
A. Chandor-Proust, O. Berteau, T. Douki, D. Gasparutto, S. Ollagnier-de-Choudens, M. Fontecave, and M. Atta (2008)
J. Biol. Chem. 283, 36361-36368
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Role of DNA Repair by Nonhomologous-End Joining in Bacillus subtilis Spore Resistance to Extreme Dryness, Mono- and Polychromatic UV, and Ionizing Radiation.
R. Moeller, E. Stackebrandt, G. Reitz, T. Berger, P. Rettberg, A. J. Doherty, G. Horneck, and W. L. Nicholson (2007)
J. Bacteriol. 189, 3306-3311
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Dinucleotide Spore Photoproduct, a Minimal Substrate of the DNA Repair Spore Photoproduct Lyase Enzyme from Bacillus subtilis.
A. Chandor, O. Berteau, T. Douki, D. Gasparutto, Y. Sanakis, S. Ollagnier-de-Choudens, M. Atta, and M. Fontecave (2006)
J. Biol. Chem. 281, 26922-26931
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Characterization of an Active Spore Photoproduct Lyase, a DNA Repair Enzyme in the Radical S-Adenosylmethionine Superfamily.
J. M. Buis, J. Cheek, E. Kalliri, and J. B. Broderick (2006)
J. Biol. Chem. 281, 25994-26003
   Abstract »    Full Text »    PDF »
Inter-strand photoproducts are produced in high yield within A-DNA exposed to UVC radiation.
T. Douki, G. Laporte, and J. Cadet (2003)
Nucleic Acids Res. 31, 3134-3142
   Abstract »    Full Text »    PDF »
Examination of Peak Power Dependence in the UV Inactivation of Bacterial Spores.
J. K. Rice and M. Ewell (2001)
Appl. Envir. Microbiol. 67, 5830-5832
   Abstract »    Full Text »    PDF »
Spore Photoproduct (SP) Lyase from Bacillus subtilis Specifically Binds to and Cleaves SP (5-Thyminyl-5,6-Dihydrothymine) but Not Cyclobutane Pyrimidine Dimers in UV-Irradiated DNA.
T. A. Slieman, R. Rebeil, and W. L. Nicholson (2000)
J. Bacteriol. 182, 6412-6417
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Resistance of Bacillus Endospores to Extreme Terrestrial and Extraterrestrial Environments.
W. L. Nicholson, N. Munakata, G. Horneck, H. J. Melosh, and P. Setlow (2000)
Microbiol. Mol. Biol. Rev. 64, 548-572
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Artificial and Solar UV Radiation Induces Strand Breaks and Cyclobutane Pyrimidine Dimers in Bacillus subtilis Spore DNA.
T. A. Slieman and W. L. Nicholson (2000)
Appl. Envir. Microbiol. 66, 199-205
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Spore Photoproduct Lyase from Bacillus subtilis Spores Is a Novel Iron-Sulfur DNA Repair Enzyme Which Shares Features with Proteins such as Class III Anaerobic Ribonucleotide Reductases and Pyruvate-Formate Lyases.
R. Rebeil, Y. Sun, L. Chooback, M. Pedraza-Reyes, C. Kinsland, T. P. Begley, and W. L. Nicholson (1998)
J. Bacteriol. 180, 4879-4885
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Ultraviolet Irradiation of DNA in vitro and in vivo Produces a Third Thymine-Derived Product.
A. J. Varghese and S. Yi Wang (1967)
Science 156, 955-957
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Pyrimidine Dimers: Effect of Temperature on Photoinduction.
R. O. Rahn (1966)
Science 154, 503-504
   Abstract »    PDF »
Thymine Addition to Ethanol: Induction by Gamma Irradiation.
P. E. Brown, M. Calvin, and J. F. Newmark (1966)
Science 151, 68-70
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The subunit structure and catalytic mechanism of the Bacillus subtilis DNA repair enzyme spore photoproduct lyase.
R. Rebeil and W. L. Nicholson (2001)
PNAS 98, 9038-9043
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