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Science 9 July 1965:
Vol. 149. no. 3680, p. 200
DOI: 10.1126/science.149.3680.200
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Articles

Bacteria as an Indicator of Formation of Antibodies by Single Spleen Cells in Agar

Stanley A. Schwartz 1 and Werner Braun 1

1 Institute of Microbiology, Rutgers University, New Brunswick, New Jersey

Bacteria can be used to measure the formation of specific antibody by suspensions of single spleen cells. The technique, based on localized immune lysis (plaque formation) in agar, permits assays on the kinetics of increase in the number of cells forming bactericidal antibody in the spleen of immunized mice. The procedure also is useful for testing nonspecific stimulation of antibody synthesis and for analyzing spleen cell populations from donors that have been immunized simultaneously with bacterial and red blood cell antigens.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Vibriolytic Antibody-Forming Cells: A New Application of the Pfeiffer Phenomenon.
R. F. McAlack, J. Cerny, J. L. Allen, and H. Friedman (1970)
Science 168, 141-142
   Abstract »    PDF »
Autoradiographic Plaques for the Detection of Antibody Formation to Soluble Proteins by Single Cells.
E. Pick and J. D. Feldman (1967)
Science 156, 964-966
   Abstract »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)