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Science 23 April 1965:
Vol. 148. no. 3669, pp. 521 - 523
DOI: 10.1126/science.148.3669.521
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Articles

Granulocytosis-Promoting Extract of Mouse Tumor Tissue: Partial Purification

L. Delmonte 1 and R. A. Liebelt 1

1 Department of Anatomy, Baylor University College of Medicine, Houston, Texas

A granulocytosis-promoting extract of mouse tumor tissue was partially purified by differential precipitation with cold 20- to 30-percent ethanol at pH 6.5, "salting out" with 50- to 75-percent saturated ammonium sulfate, and filtration through Sephadex G-75 dextran gel. In intact mice, single doses of the extract (at least 12 micrograms per gram of body weight) induced up to a 30-fold increase in circulating granulocytes and a two- to threefold decrease in circulating mononuclear cells within 3 to 7 hours of intravenous injection.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)