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Science 26 March 1965:
Vol. 147. no. 3665, pp. 1578 - 1579
DOI: 10.1126/science.147.3665.1578
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Articles

Agrobacterium tumefaciens: Thermal Inactivation of Tumor-Inducing Ability

James A. Lippincott 1 and Barbara B. Lippincott 1

1 Department of Biological Sciences, Northwestern University, Evanston, Illinois

Treating cultures of Agrobacterium tumefaciens at 39° to 48°C reduces the infectivity of the bacteria without necessarily affecting viability. Destruction of the capacity to initiate tumor growth follows first-order kinetics from which rate constants for thermal inactivation are derived. From these rates, values for heat of activation of 56.7 kcal mole-1 and entropy of activation of 107 cal mole-1 deg-1 are obtained. A particular protein or nucleoprotein active in the process of infection may be inactivated by the treatment.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)