Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 19 March 1965:
Vol. 147. no. 3664, pp. 1454 - 1455
DOI: 10.1126/science.147.3664.1454
Prev | Table of Contents | Next

Articles

Growth and Tissue Formation from Single, Isolated Tobacco Cells in Microculture

Vimla Vasil 1 and A. C. Hildebrandt 1

1 Department of Plant Pathology, University of Wisconsin, Madison

Single, isolated cells of tobacco divided and grew to form small colonies of over 50 cells in microcultures with a medium containing fresh liquid coconut milk, but in the absence of neighboring cells or nurse tissue. Subsequently, the mass of cells obtained from the single cell, when transferred to agar coconut milk medium, established itself as a clone of callus tissue. Some of these single-cell clones showed differentiation of many tracheid-like cells and shoots with small leaves.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The Scientific Roots of Modern Plant Biotechnology.
I. M. Sussex (2008)
PLANT CELL 20, 1189-1198
   Full Text »    PDF »
Growth in Microculture of Single Tobacco Cells Infected with Tobacco Mosaic Virus.
N. Chandra and A. C. Hildebrandt (1966)
Science 152, 789-791
   Abstract »    PDF »
Differentiation of Tobacco Plants from Single, Isolated Cells in Microcultures.
V. Vasil and A. C. Hildebrandt (1965)
Science 150, 889-892
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)