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Science 11 December 1964:
Vol. 146. no. 3650, pp. 1476 - 1477
DOI: 10.1126/science.146.3650.1476
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Articles

Bone Cells: Biochemical and Biological Studies after Enzymatic Isolation

William A. Peck 1, Stanley J. Birge Jr. 1, and Susan A. Fedak 1

1 National Institute of Arthritis and Metabolic Diseases, Bethesda 14, Maryland

Short-term incubation of rat calvaria in buffered crude collagenase permitted the isolation of morphologically intact cells that absorb vital dyes, contain alkaline phosphatase, and multiply in tissue culture. Freshly harvested cells were similar to whole bone segments in aerobic glucose metabolism.


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Science. ISSN 0036-8075 (print), 1095-9203 (online)