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Science 20 November 1964:
Vol. 146. no. 3647, pp. 1039 - 1043
DOI: 10.1126/science.146.3647.1039
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Articles

Osmiophilic Reagents: New Cytochemical Principle for Light and Electron Microscopy

Jacob S. Hanker 1, Arlene R. Seaman 1, Leon P. Weiss 1, Hiromi Ueno 1, Ronald A. Bergman 1, and Arnold M. Seligman 1

1 Department of Surgery, Sinai Hospital of Baltimore and the Departments of Surgery and Anatomy, Johns Hopkins University School of Medicine, Baltimore, Maryland

Established histochemical procedures have been altered by utilizing reagents which contain a group capable of reacting selectively with OsO4. This permits the cytochemical demonstration of a particular enzyme or functional group of a macromolecule with the light and electron microscope. The osmiophilic groups used thus far are mercapto, thiocarbamyl, and diazothioether. The "osmium black" produced at the site of reaction is electron opaque, is insoluble in lipids, and is not altered when the issue is embedded in acrylic and epoxy resins. Its fine amorphous character enables it to be maintained precisely in the electron beam.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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Osmiophilic Polymer Generation: Catalysis by Transition Metal Compounds in Ultrastructural Cytochemistry.
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Carcinogens 3,4-Benzpyrene and 3-Methylcholanthrene: Induction of Mitochondrial Oxidative Enzymes.
N. Zenker, J. S. Hanker, Y. Morizono, C. Deb, and A. M. Seligman (1968)
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Coordination Polymers of Osmium: The Nature of Osmium Black.
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Science 156, 1737-1738
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Staining Tissue for Light and Electron Microscopy by Bridging Metals with Multidentate Ligands.
J. S. Hanker, C. Deb, H. L. Wasserkrug, and A. M. Seligman (1966)
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Science. ISSN 0036-8075 (print), 1095-9203 (online)