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Science 23 October 1964:
Vol. 146. no. 3643, pp. 533 - 535
DOI: 10.1126/science.146.3643.533
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Articles

5-Bromodeoxyuridine: Effect on Myogenesis in vitro

F. Stockdale 1, K. Okazaki 1, M. Nameroff 1, and H. Holtzer 1

1 Department of Anatomy, School of Medicine, University of Pennsylvania, Philadelphia

Presumptive myoblasts, obtained by treating muscle from 11-day chick embryos with trypsin, multiply in vitro. On the 4th or 5th day in culture they abruptly fuse, form long multinucleated myotubes, and begin to synthesize myosin. Cultured cells exposed to 5-bromodeoxyuridine incorporate this analog of thymidine into their DNA. Cells with such falsified DNA are reversibly inhibited from forming myotubes and synthesizing myosin; such cells, however, continue to synthesize the various species of molecules required for cell multiplication.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Embryonic and Postnatal Injections of Bromodeoxyuridine Produce Age-Dependent Morphological and Behavioral Abnormalities.
B. Kolb, B. Pedersen, M. Ballermann, R. Gibb, and I. Q. Whishaw (1999)
J. Neurosci. 19, 2337-2346
   Abstract »    Full Text »    PDF »
5-Bromo-2-deoxyuridine regulates invasiveness and expression of integrins and matrix-degrading proteinases in a differentiated hamster melanoma cell.
L Thomas, P. Chan, S Chang, and C Damsky (1993)
J. Cell Sci. 105, 191-201
   Abstract »    PDF »
The myoD gene family: nodal point during specification of the muscle cell lineage.
H Weintraub, R Davis, S Tapscott, M Thayer, M Krause, R Benezra, T. Blackwell, D Turner, R Rupp, S Hollenberg, et al. (1991)
Science 251, 761-766
   Abstract »    PDF »
5-bromo-2'-deoxyuridine blocks myogenesis by extinguishing expression of MyoD1.
S. Tapscott, A. Lassar, R. Davis, and H Weintraub (1989)
Science 245, 532-536
   Abstract »    PDF »
Superinduction of Endogenous Type C Virus by 5-Bromodeoxyuridine from Transformed Mouse Clones.
M. M. Lieber, D. M. Livingston, and G. J. Todaro (1973)
Science 181, 443-444
   Abstract »    PDF »
Selected Topics in Skeletal Myogenesis.
H. Holtzer, J. W. Sanger, H. Ishikawa, and K. Strahs (1973)
Cold Spring Harb Symp Quant Biol 37, 549-566
   Abstract »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)