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Science 16 October 1964:
Vol. 146. no. 3642, pp. 407 - 408
DOI: 10.1126/science.146.3642.407
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Articles

Actinomycin Resistance in Bacillus subtilis

Irving J. Slotnick 1 and Bruce H. Sells 1

1 Laboratories of Bacteriology and Biochemistry, St. Jude Research Hospital, Memphis, Tennessee

The incorporation of uracil-C14, a precursor of cellular RNA, into whole cells of the sensitive parent strain Bacillus subtilis No. 6051 is completely inhibited by actinomycin D, whereas only partial inhibition of uracil-14 incorporation occurs with actinomycin-resistant intact cells of B. subtilis strain II 15. Incorporation of uracil-C14 into protoplasts of the resistant strain of B. subtilis is completely inhibited. The sensitivity of resistant-cell protoplasts to drug effect clearly supports the conclusion that actinomycin penetration is altered in resistant intact whole cells. Since other experiments have shown that the antibiotic is not broken down in the presence of resistant cells, resistance is considered to result from a change in cell permeability.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Incorporation of Tritiated Actinomycin D into Drug-Sensitive and Drug-Resistant HeLa Cells.
M. N. Goldstein, K. Hamm, and E. Amrod (1966)
Science 151, 1555-1556
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Science. ISSN 0036-8075 (print), 1095-9203 (online)