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Science 18 September 1964:
Vol. 145. no. 3638, pp. 1313 - 1315
DOI: 10.1126/science.145.3638.1313
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Articles

Intracellular Perfusion of Chilean Giant Squid Axons

Ichiji Tasaki 1 and Mario Luxoro 2

1 Laboratory of Neurobiology, National Institutes of Health, Bethesda, Marylatnd 20014
2 Institute of Science, University of Chile, Santiago

Axons of the Chilean giant squid were subjected to continuous intracellular perfusion with either potassium- or sodium-rich salt solutions. In axons immersed in natural sea water and internally perfused with potassium glutamate solution, action potentials which exceeded 160 millivolts were often observed. The resting potential did not vary appreciably with the internal concentration of potassium glutamate. With perfusing solutions containing sodium aspartate or sodium glutamate, action potentials were observed which exceeded the value calculated from the Nernst equation applied to sodium-ion activity. Nervous conduction could be maintained more than 1 hour with equal concentrations of sodium inside and outside the axon.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Electrophysiological Studies of Chilean Squid Axons under Internal Perfusion with Sodium-Rich Media.
I. Tasaki, M. Luxoro, and A. Ruarte (1965)
Science 150, 899-901
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Science. ISSN 0036-8075 (print), 1095-9203 (online)