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Science 29 May 1964:
Vol. 144. no. 3622, pp. 1142 - 1144
DOI: 10.1126/science.144.3622.1142
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Articles

DNA from Bacteriophage Lambda: Molecular Length and Conformation

L. A. MacHattie 1 and C. A. Thomas Jr. 1

1 Biophysics Department, Johns Hopkins University, Baltimore, Maryland

The length of the DNA molecules from wild-type lgr bacteriophage is 17.2 µ, corresponding to a molecular weight of 33 million in the B form. The molecules from a double-deletion mutant of lgr are 23 percent shorter. Both types of molecules join ends at 60°C to form circular molecules or polymers. The point of junction cannot be distinguished by any irregularity in the uniform duplex molecule.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Bacteriophage T7.
F. W. Studier (1972)
Science 176, 367-376
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Mapping of Deletions and Substitutions in Heteroduplex DNA Molecules of Bacteriophage Lambda by Electron Microscopy.
B. C. Westmoreland, W. Szybalski, and H. Ris (1969)
Science 163, 1343-1348
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Breakage of DNA in Rec+ and Rec- Bacteria by Disintegration of Radiophosphorous Atoms in DNA and Possible Cause of Pleiotropic Effects of RecA Mutation.
J.-i. Tomizawa and H. Ogawa (1968)
Cold Spring Harb Symp Quant Biol 33, 243-251
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Studies with Escherichia coli Sex Factors.
D. Freifelder (1968)
Cold Spring Harb Symp Quant Biol 33, 425-434
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Chemistry and Structure of Nucleic Acids of Bacteriophages: Many forms of nucleic acids of bacteriophages show the ways that information is stored and reproduced.
J. A. Cohen (1967)
Science 158, 343-351
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Correlation between Location and Time of Expression for Genes in a Single Operon.
D. Marver, M. A. Berbevich, and R. F. Golderger (1966)
Science 153, 1655-1656
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Tritium and Phosphorus-32 in High-Resolution Autoradiography.
L. G. Caro and M. Schnos (1965)
Science 149, 60-62
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Science. ISSN 0036-8075 (print), 1095-9203 (online)