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Science 24 January 1964:
Vol. 143. no. 3604, pp. 376 - 378
DOI: 10.1126/science.143.3604.376
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Articles

Hybridization of Half Molecules of Rabbit Gamma Globulin

A. Nisonoff 1 and J. L. Palmer 1

1 Department of Microbiology, University of Illinois, Urbana

Specifically purified rabbit antiovalbumin and normal ggr-globulin labeled with 1-131 were dissociated into half molecules by reduction and acidification. When a mixture of the two preparations was neutralized, a large proportion of mixed molecules having active combining sites and the same sedimentation coefficient as the original ggr-globulins was formed. Since the sulfhydryl groups were inactivated after reduction, the recombined subunits appear to be linked by noncovalent bonds.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Lattice Formation in Complement Fixation: Studies with Univalent Rabbit Antibody.
H. H. Fudenberg, R. Hong, and A. Nisonoff (1965)
Science 148, 91-93
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Science. ISSN 0036-8075 (print), 1095-9203 (online)