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Science 27 December 1963:
Vol. 142. no. 3600, pp. 1654 - 1655
DOI: 10.1126/science.142.3600.1654
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Articles

Ethylene Oxide Sterilization of Tissue Culture Media

Burke L. Brown 1 and Robert Fuerst 1

1 Department of Biology, Texas Woman's University, Denton

Liquid ethylene oxide, which kills bacteria, yeasts, and molds, was added to a tissue culture medium instead of the usual antibiotics. With some limitations, the use of ethylene oxide sterilization of tissue culture medium has been shown feasible. Data indicate that this method can be used in experiments involving the sterilization of test chemicals and components of the medium used for mammalian cells in culture.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)