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Science 8 November 1963:
Vol. 142. no. 3593, pp. 674 - 676
DOI: 10.1126/science.142.3593.674
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Articles

Binding Capacity of Reductively Fragmented Antibodies to the 2,4-Dinitrophenyl Group

H. Metzger 1 and S. J. Singer 1

1 Department of Biology, University of California, La Jolla

Mild reductive cleavage of the polypeptide chains of antibodies to the 2,4-dinitrophenyl group has been carried out according to the methods of Porter and co-workers. The addition of egr-2,4-dinitrophenyllysine permitted almost complete retention of the antibody binding capacity upon transfer to IM propionic acid. Fractionation of the chains showed that the hapten was specifically bound to the A and not the B chains, and that about 30 percent of the B chains were bound to A in the presence of the bound hapten. The results confirm the conclusion of Porter and ca-workers that the A chain is the principal component of the antibody site, but suggest that the B chain may also contribute to the site.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Antibody Active Sites and Immunoglobulin Molecules.
S. J. Singer and R. F. Doolittle (1966)
Science 153, 13-25
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Science. ISSN 0036-8075 (print), 1095-9203 (online)