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Science 18 October 1963:
Vol. 142. no. 3590, pp. 387 - 389
DOI: 10.1126/science.142.3590.387
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Articles

Transcription in vivo of DNA from Bacteriophage SP8

J. Marmur 1 and C. M. Greenspan 1

1 Department of Biochemistry, Brandeis University, Waltham, Massachusetts

The DNA of bacteriophage SP8, when denatured, yields two components differing in buoyant density in cesium chloride gradients and separable by chromatography on a column of methylated bovine serum albumin and kieselguhr. The denser of the two strands (H) contains more pyrimidines and fewer purines than the lighter (L) strand. Only the H strand forms hybrids with the RNA synthesized by the infected host. The L strand is capable of annealing with complementary RNA synthesized in vitro with it as primer in reactions catalyzed by RNA polymerase. During the vegetative development of phage, host-specific messenger RNA is also synthesized.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
A Molecular Approach in Systematics of Higher Organisms.
B. H. Hoyer, B. J. McCarthy, and E. T. Bolton (1964)
Science 144, 959-967
   PDF »
Specificity of the Complementary RNA Formed by Bacillus subtilis Infected with Bacteriophage SP8.
J. Marmur, C. M. Greenspan, E. Palecek, F. M. Kahan, J. Levine, and M. Mandel (1963)
Cold Spring Harb Symp Quant Biol 28, 191-199
   Abstract »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)