Electron Microscope Autoradiography of Bacteria Labeled with Iodine-125
Nobuko O. Kuhn 1 and
Carl G. Harford 1
1 Department of Medicine, Washington University School of Medicine, St. Louis 10, Missouri
The low-energy extranuclear electrons emitted by iodine-125 can be used for electron microscope autoradiography with results comparable to those obtained with tritium. Autoradiographs of tritium-labeled bacteria showed that 71 percent of all reacted grains localized on the cell. This figure was 86 percent when I125-labeled bacteria were used.