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Science 21 June 1963:
Vol. 140. no. 3573, pp. 1313 - 1316
DOI: 10.1126/science.140.3573.1313
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Articles

Replicating Form of a Single-Stranded DNA Virus: Isolation and Properties

M. Hayashi 1, M. N. Hayashi 1, and S. Spiegelman 1

1 Department of Microbiology, University of Illinois, Urbana

The replicating form of single-stranded DNA virus has been isolated in pure form by chromatography on columns of methylated albumin. Its buoyant density in CsCl and "melting temperature" are characteristic of a double stranded DNA structure containing 43 percent guanine-cytosine. The nearest neighbors to uridylate were compared in the RNA synthesized when replicating-form DNA and mature single-stranded DNA were employed as templates in an in vitro system. The mature DNA component of the replicating duplex does not serve as the sole source of complementary RNA. The results agree best with the assumption that both strands of the replicating form function as templates. It is important to note that this is contrary to the situation found in the intact cell where only one of the two strands appears to be transcribed into message.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Circularity of the Replicating Form of a Single-Stranded DNA Virus.
B. Chandler, M. Hayashi, M. N. Hayashi, and S. Spiegelman (1964)
Science 143, 47-49
   Abstract »    PDF »
The Present Status of the Transfer of Genetic Information and Its Control.
S. Spiegelman and M. Hayashi (1963)
Cold Spring Harb Symp Quant Biol 28, 161-181
   Abstract »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)