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Science 19 April 1963:
Vol. 140. no. 3564, pp. 290 - 292
DOI: 10.1126/science.140.3564.290
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Articles

In vitro Culture of the Flagellate Protozoan Hexamita salmonis

J. R. Uzmann 1 and S. Hobbs Hayduk 1

1 U. S. Fish and Wildlife Service, Western Fish Disease Laboratory, Seattle 15, Washington

Trophozoites of Hexamita salmonis, asserted pathogen of juvenile salmonid fishes, were isolated from two species of Pacific salmon hosts and cultured repeatedly in an organic medium saturated with nitrogen. Primary isolates and serial subcultures usually exhibited five- to tenfold population increases per passage.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)