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Science 12 April 1963:
Vol. 140. no. 3563, pp. 180 - 183
DOI: 10.1126/science.140.3563.180
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Articles

Breakdown of Rat-Liver Ergosomes in vivo after Actinomycin Inhibition of Messenger RNA Synthesis

Theophil Staehelin 1, Felix O. Wettstein 1, and Hans Noll 1

1 Department of Microbiology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania

Ribosomes isolated from rat liver occur predominantly in the form of aggregates (ergosomes) corresponding to multiples of 73S particles held together by messenger RNA. After injecting rats with actinomycin, these aggregates gradually break down in vivo to 73S monomers and 113S dimers. We conclude that the observed breakdown results from the degradation of messenger RNA and the prevention by actinomycin of the synthesis of new messenger RNA.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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Actinomycin D: Inhibition of Protein Synthesis Unrelated to Effect on Template RNA Synthesis.
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Actinomycin D: An Effect on Rat Liver Homogenates Unrelated to Its Action on RNA Synthesis.
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Antibody Formation in vitro by Separated Spleen Cells: Inhibition by Actinomycin or Chloramphenicol.
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The Structure and Function of Polyribosomes.
A. Rich, J. R. Warner, and H. M. Goodman (1963)
Cold Spring Harb Symp Quant Biol 28, 269-285
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