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Science 16 November 1962:
Vol. 138. no. 3542, pp. 813 - 817
DOI: 10.1126/science.138.3542.813
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Articles

Fate of a Synthetic Polynucleotide Directing Cell-Free Protein Synthesis II. Association with Ribosomes

Samuel H. Barondes 1 and Marshall W. Nirenberg 1

1 National Institutes of Health, Bethesda, Maryland

Tritiated-poly U, when added to cell-free extracts of Escherichia coli, became associated with ribosomes. This association occurred at 3°C and did not require high-energy phosphate compounds. Small amounts of tritiated polyuridylic acid produced polydisperse ribosomal aggregates with sedimentation constants of approximately 100 to 130. C14-phenylalanine initially was incorporated into protein only on these particles, which suggests that they are the sites of polyphenylalanine synthesis.


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