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Science 19 October 1962:
Vol. 138. no. 3538, pp. 440 - 442
DOI: 10.1126/science.138.3538.440
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Articles
Localizing Tritiated Norepinephrine in Sympathetic Axons by Electron Microscopic Autoradiography
D. E. Wolfe 1,
L. T. Potter 1,
K. C. Richardson 1, and
J. Axelrod 1
1 National Institute of Neurological Diseases and Blindness, and National Institute of Mental Health, Bethesda, Maryland
Following intravenous infusion of tritiated norepinephrine, rat pineals were prepared for combined autoradiography and electron microscopy. Concentrations of photographic grains were observed only over regions of preterminal autonomic axons containing granulated vesicles, thereby directly demonstrating uptake of norepinephrine into these axons and strongly suggesting that their granulated vesicles contain norepinephrine.
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