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Science 4 August 1961:
Vol. 134. no. 3475, pp. 336 - 337
DOI: 10.1126/science.134.3475.336
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Articles

Axenic Cultivation of Entamoeba histolytica

Louis S. Diamond 1

1 Laboratory of Parasitic Diseases, National Institutes of Health, Bethesda, Maryland

Entamoeba histolytica (National Institutes of Health strain 200) has been maintained in axenic culture through 50 transfers over a period of 6 months. The medium used is diphasic and contains a cell-free extract of chick embryo which is essential for growth. Inocula of 50,000 amoebas yield 150,000 to 200,000 organisms in 72 hours of incubation at 35°C. An extract prepared from boiled embryos supports growth equally well.


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Eukaryot. Cell 7, 1565-1572
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Laboratory Diagnostic Techniques for Entamoeba Species.
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Overexpression of myosin IB in living Entamoeba histolytica enhances cytoplasm viscosity and reduces phagocytosis.
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Laboratory Diagnosis of Amebiasis.
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Methods for Cultivation of Luminal Parasitic Protists of Clinical Importance.
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Myosin IB from Entamoeba histolytica is involved in phagocytosis of human erythrocytes.
H Voigt, J. Olivo, P Sansonetti, and N Guillen (1999)
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Cloning and Molecular Characterization of a cDNA Clone Coding for Trichomonas vaginalis Alpha-Actinin and Intracellular Localization of the Protein.
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Virulence and Functions of Myosin II Are Inhibited by Overexpression of Light Meromyosin in Entamoeba histolytica.
P. Arhets, J.-C. Olivo, P. Gounon, P. Sansonetti, and N. Guillén (1998)
Mol. Biol. Cell 9, 1537-1547
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Collection, Identification, and Cultivation of Oral Protozoa.
W. W. Wantland, E. M. Wantland, and D.L. Winquist (1963)
Journal of Dental Research 42, 1234-1241
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