Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 23 December 1960:
Vol. 132. no. 3443, pp. 1890 - 1891
DOI: 10.1126/science.132.3443.1890
Prev | Table of Contents | Next

Articles

Preparation of Monolayer Cell Cultures from Tissues of Some Lower Vertebrates

Ken Wolf 1, M. C. Quimby 1, E. A. Pyle 1, and R. P. Dexter 1

1 Bureau of Sport Fisheries and Wildlife, Eastern Fish Disease Laboratory, Leetown (P. O. Kearneysville), West Virginia

Cold trypsin dispersion at pH 7.2 was used to obtain cultivable cells and cell groups from tissues of six species of fresh-water bony fishes, a frog, and a turtle. The cells readily attached to glass and were capable of at least limfted, and in some cases extended, division in media consisting of commercially available components.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Vertebrate Regeneration System: Culture in vitro.
S. B. Simpson Jr. and P. G. Cox (1967)
Science 157, 1330-1332
   Abstract »    PDF »
Amphibian Cell Culture: Permanent Cell Line from the Bullfrog (Rana catesbeiana).
K. Wolf and M. C. Quimby (1964)
Science 144, 1578-1580
   Abstract »    PDF »
Established Eurythermic Line of Fish Cells in vitro.
K. Wolf and M. C. Quimby (1962)
Science 135, 1065-1066
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)