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Science 9 December 1960:
Vol. 132. no. 3441, pp. 1770 - 1771
DOI: 10.1126/science.132.3441.1770
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Articles

Properties of the Major Component of a Peptic Digest of Rabbit Antibody

A. Nisonoff 1, F. C. Wissler 1, and L. N. Lipman 1

1 Departments of Biochemistry and Biophysics, Roswell Park Memorial Institute, Buffalo, New York

The molecular weight of the active, major component isolated from a peptic digest of rabbit antibody was found to be 106,000. After treatment with a disulfide-splitting reagent, the molecular weight was 56,000, and the products migrated as a single peak in the ultracentrifuge. he univalent fragments thus formed can be partially recombined by passage through IR-120 cation-exchange resin at room temperature or by treatment with a difunctional organic mercurial. Some splitting of the pepsin-treated antibody molecule occurs on carboxymethylcellulose at pH 5.4.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Functional Characterization of Rabbit Lymphocytes Carrying Fc Receptor.
P.-A. Cazenave, D. Juy, and C. Bona (1977)
Cold Spring Harb Symp Quant Biol 41, 307-314
   Abstract »    PDF »
Antibody to Rat Kidney: In vivo Effects of Univalent and Divalent Fragments.
J. H. Baxter and P. A. Small Jr. (1963)
Science 140, 1406-1407
   Abstract »    PDF »


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