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Science 8 July 1960:
Vol. 132. no. 3419, pp. 86 - 87
DOI: 10.1126/science.132.3419.86
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Articles

Isolation of Antibodies to Gelatin from Antigen-Antibody Complex by Proteolysis

Ruth Arnon 1 and Michael Sela 1

1 Department of Biophysics, Weizmann Institute of Science, Rehovoth, Israel

Antibodies were isolated by digestion of the gelatin-antigelatin complex with collagenase and removal of the gelatin fragments by dialysis. The pure antibody could be precipitated anew by the antigen. Antibodies to tyrosylgelatin were isolated in a similar way, but in this case peptides containing the active antigenic sites remained bound to the antibody.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
From Proteins and Protein Models to Their Use in Immunology and Immunotherapy.
M. Sela (2003)
J. Biol. Chem. 278, 48507-48519
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Catgut Allergy in Eye Muscle Surgery: II. Correlation and Comparison of Eye Reaction and Skin Test After the Use of Plain and Chromicized Catgut.
L. APT, F. D. COSTENBADER, M. M. PARKS, and D. G. ALBERT (1961)
Arch Ophthalmol 65, 474-480
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Science. ISSN 0036-8075 (print), 1095-9203 (online)