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Science 30 January 1959:
Vol. 129. no. 3344, pp. 274 - 275
DOI: 10.1126/science.129.3344.274
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Articles

Purification of Folic Acid

WARWICK SAKAMI 1 and ROBERT KNOWLES 1

1 Department of Biochemistry, Western Reserve University School of Medicine, Cleveland, Ohio

Various purification procedures for folic acid were investigated. The criteria of purity were a negative Bratton-Marshall test and the absence of fluorescent spots on paper chromatograms. Since no method provided a pure product, a procedure consisting of cellulose column chromatography followed by filtration through charcoal was developed.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)