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Science 26 December 1958:
Vol. 128. no. 3339, pp. 1625 - 1626
DOI: 10.1126/science.128.3339.1625
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Articles

Measurement of Precipitin Reactions in the Millimicrogram Protein-Nitrogen Range

DAVID GLICK 1, ROBERT A. GOOD 1, LEONARD J. GREENBERG 1, JENNIFER J. EDDY 1, and NOORBIBI K. DAY 1

1 Histochemistry Laboratory, Department of Physiological Chemistry, and Department of Pediatrics, University of Minnesota Medical School, Minneapolis

Exploitation of newer instrumentation and a dye-binding method for protein measurement made possible reduction in volume down to 1/1000 that commonly used, with no greater error. The procedure was tested at two levels, 1- and 10-µl volumes, with human gamma globulin and rabbit antiserum. Of the dyes tested, bromsulfalein proved best for the protein estimation.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)