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Originally published in Science Express on 6 September 2007
Science 5 October 2007:
Vol. 318. no. 5847, pp. 71 - 76
DOI: 10.1126/science.1146221
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Research Articles

A Neuroligin-3 Mutation Implicated in Autism Increases Inhibitory Synaptic Transmission in Mice

Katsuhiko Tabuchi,1 Jacqueline Blundell,2 Mark R. Etherton,1 Robert E. Hammer,3 Xinran Liu,1 Craig M. Powell,2,4 Thomas C. Südhof1,5,6*

Autism spectrum disorders (ASDs) are characterized by impairments in social behaviors that are sometimes coupled to specialized cognitive abilities. A small percentage of ASD patients carry mutations in genes encoding neuroligins, which are postsynaptic cell-adhesion molecules. We introduced one of these mutations into mice: the Arg451->Cys451 (R451C) substitution in neuroligin-3. R451C mutant mice showed impaired social interactions but enhanced spatial learning abilities. Unexpectedly, these behavioral changes were accompanied by an increase in inhibitory synaptic transmission with no apparent effect on excitatory synapses. Deletion of neuroligin-3, in contrast, did not cause such changes, indicating that the R451C substitution represents a gain-of-function mutation. These data suggest that increased inhibitory synaptic transmission may contribute to human ASDs and that the R451C knockin mice may be a useful model for studying autism-related behaviors.

1 Department of Neuroscience, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
2 Department of Neurology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
3 Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
4 Department of Psychiatry, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
5 Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
6 Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.

* To whom correspondence should be addressed. E-mail: thomas.sudhof{at}utsouthwestern.edu

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