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Published Online April 26, 2007
Science DOI: 10.1126/science.1142950

Reports

Submitted on March 23, 2007
Accepted on April 17, 2007

Revisiting the Role of the Mother Centriole in Centriole Biogenesis

A. Rodrigues-Martins 1, M. Riparbelli 2, G. Callaini 2, D. M. Glover 3*, M. Bettencourt-Dias 1*

1 Instituto Gulbenkian de Ciência, Cell Cycle Regulation Lab, Rua da Quinta Grande, 6, P-2780-156 Oeiras, Portugal; Cancer Research UK Cell Cycle Genetics Research Group, Department of Genetics, University of Cambridge, Downing Street, Cambridge, CB2 3EH, United Kingdom.
2 Department of Evolutionary Biology, University of Siena, Via A. Moro 4, I-53100 Siena, Italy.
3 Cancer Research UK Cell Cycle Genetics Research Group, Department of Genetics, University of Cambridge, Downing Street, Cambridge, CB2 3EH, United Kingdom.

* To whom correspondence should be addressed.
D. M. Glover , E-mail: dmg25{at}hermes.cam.ac.uk
M. Bettencourt-Dias , E-mail: mdias{at}igc.gulbenkian.pt

Centrioles duplicate once in each cell division cycle through so- called templated or canonical duplication. SAK/PLK4, a kinase implicated in tumor development, is an upstream regulator of canonical biogenesis necessary for centriole formation. Here we found that overexpression of SAK/PLK4 could induce amplification of centrioles in Drosophila embryos and their de novo formation in unfertilised eggs. Both processes required the activity of DSAS-6 and DSAS-4, two molecules required for canonical duplication. Thus centriole biogenesis is a template-free self-assembly process triggered and regulated by molecules that ordinarily associate with the existing centriole. The mother centriole is not a bona-fide "template" but a platform for a set of regulatory molecules that catalyzes and regulates daughter centriole assembly.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
ECRG2 Disruption Leads to Centrosome Amplification and Spindle Checkpoint Defects Contributing Chromosome Instability.
X. Cheng, Z. Shen, J. Yang, S.-H. Lu, and Y. Cui (2008)
J. Biol. Chem. 283, 5888-5898
   Abstract »    Full Text »    PDF »
SAS-4 is recruited to a dynamic structure in newly forming centrioles that is stabilized by the {gamma}-tubulin-mediated addition of centriolar microtubules.
A. Dammermann, P. S. Maddox, A. Desai, and K. Oegema (2008)
J. Cell Biol. 180, 771-785
   Abstract »    Full Text »    PDF »
The Golgi Protein GM130 Regulates Centrosome Morphology and Function.
A. Kodani and C. Sutterlin (2008)
Mol. Biol. Cell 19, 745-753
   Abstract »    Full Text »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)