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Originally published in Science Express on 14 December 2006
Science 26 January 2007:
Vol. 315. no. 5811, pp. 482 - 486
DOI: 10.1126/science.1133542
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Research Articles

Histocompatible Embryonic Stem Cells by Parthenogenesis

Kitai Kim,1,2,4 Paul Lerou,1,4,5 Akiko Yabuuchi,1,2,4 Claudia Lengerke,1,2,4 Kitwa Ng,1,2,4 Jason West,1,2,4 Andrew Kirby,6 Mark J. Daly,6 George Q. Daley1,2,3,4*

Genetically matched pluripotent embryonic stem (ES) cells generated via nuclear transfer or parthenogenesis (pES cells) are a potential source of histocompatible cells and tissues for transplantation. After parthenogenetic activation of murine oocytes and interruption of meiosis I or II, we isolated and genotyped pES cells and characterized those that carried the full complement of major histocompatibility complex (MHC) antigens of the oocyte donor. Differentiated tissues from these pES cells engrafted in immunocompetent MHC-matched mouse recipients, demonstrating that selected pES cells can serve as a source of histocompatible tissues for transplantation.

1 Division of Pediatric Hematology/Oncology, Children's Hospital Boston and Dana Farber Cancer Institute, Boston, MA 02115, USA.
2 Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA.
3 Division of Hematology, Brigham and Women's Hospital, Boston, MA 02115, USA.
4 Harvard Stem Cell Institute, Cambridge, MA 02138, USA.
5 Division of Newborn Medicine, Brigham and Women's Hospital and Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.
6 Center for Human Genetic Research, Massachusetts General Hospital, Boston, MA 02115, USA.

* To whom correspondence should be addressed. E-mail: george.daley{at}childrens.harvard.edu

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Heterozygous Embryonic Stem Cell Lines Derived from Nonhuman Primate Parthenotes.
V. Dighe, L. Clepper, D. Pedersen, J. Byrne, B. Ferguson, S. Gokhale, M. C. T. Penedo, D. Wolf, and S. Mitalipov (2008)
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Towards the Generation of Patient-Specific Pluripotent Stem Cells for Combined Gene and Cell Therapy of Hematologic Disorders.
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Science. ISSN 0036-8075 (print), 1095-9203 (online)