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Originally published in Science Express on 1 September 2005
Science 23 September 2005:
Vol. 309. no. 5743, pp. 2064 - 2067
DOI: 10.1126/science.1114758
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Reports

Direct Isolation of Satellite Cells for Skeletal Muscle Regeneration

Didier Montarras,1* Jennifer Morgan,3,4 Charlotte Collins,4 Frédéric Relaix,1 Stéphane Zaffran,1 Ana Cumano,2 Terence Partridge,4 Margaret Buckingham1*

Muscle satellite cells contribute to muscle regeneration. We have used a Pax3GFP/+ mouse line to directly isolate (Pax3)(green fluorescent protein)–expressing muscle satellite cells, by flow cytometry from adult skeletal muscles, as a homogeneous population of small, nongranular, Pax7+, CD34+, CD45–, Sca1– cells. The flow cytometry parameters thus established enabled us to isolate satellite cells from wild-type muscles. Such cells, grafted into muscles of mdx nu/nu mice, contributed both to fiber repair and to the muscle satellite cell compartment. Expansion of these cells in culture before engraftment reduced their regenerative capacity.

1 CNRS Unité de Recherche Associée 2578, Department of Developmental Biology
2 Unité du Développement des Lymphocytes, Unité 668, INSERM, Pasteur Institute, 75724 Paris Cedex 15, France.
3 Department of Paediatrics, Imperial College London, The Dubowitz Neuromuscular Centre, Hammersmith Hospital, Du Cane Road, London W12 ONN, UK.
4 Muscle Cell Biology Group, Medical Research Council Clinical Sciences Centre, Imperial College, Du Cane Road, London W12 ONN, UK.

* To whom correspondence should be addressed. E-mail: dmontarr{at}pasteur.fr (D.M.); margab{at}pasteur.fr (M.B.)

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