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Published Online May 19, 2005
Science DOI: 10.1126/science.1112286

Reports

This article has been retracted

Submitted on March 15, 2005
Accepted on May 12, 2005

Patient-Specific Embryonic Stem Cells Derived from Human SCNT Blastocysts

Woo Suk Hwang 1*, Sung Il Roh 2, Byeong Chun Lee 3, Sung Keun Kang 3, Dae Kee Kwon 3, Sue Kim 3, Sun Jong Kim 2, Sun Woo Park 3, Hee Sun Kwon 3, Chang Kyu Lee 4, Jung Bok Lee 2, Jin Mee Kim 2, Curie Ahn 5, Sun Ha Paek 5, Sang Sik Chang 6, Jung Jin Koo 6, Hyun Soo Yoon 7, Jung Hye Hwang 7, Youn Young Hwang 7, Ye Soo Park 7, Sun Kyung Oh 5, Hee Sun Kim 5, Jong Hyuk Park 8, Shin Yong Moon 5, Gerald Schatten 8*

1 College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea; School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea.
2 Medical Research Center, MizMedi Hospital, Seoul, 135-280, Korea.
3 College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
4 School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea.
5 College of Medicine, Seoul National University, Seoul, 110-744, Korea.
6 Hanna Women's Clinic, Seoul, 137-872, Korea.
7 School of Medicine, Hanyang University, Seoul, 471-701, Korea.
8 Pittsburgh Development Center, Magee-Womens Research Institute, Departments of Obstetrics-Gynecology-Reproductive Sciences and Cell Biology-Physiology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213 USA.

* To whom correspondence should be addressed.
Woo Suk Hwang , E-mail: hwangws{at}snu.ac.kr
Gerald Schatten , E-mail: gschatten{at}pdc.magee.edu

Patient-specific, immune-matched human embryonic stem cells (hESC) are anticipated to be of great biomedical importance for studies of disease and development, and to advance clinical deliberations for stem cell transplantation. Eleven hESC lines were established by nuclear transfer (SCNT; NT) of skin cells from patients with disease or injury into donated oocytes. These lines (NT-hESCs), grown on human feeders from the same NT-donor or genetically-unrelated individuals, were established at high rates, regardless of NT-donor sex or age. NT-hESCs were pluripotent, chromosomally normal, and match NT-patient's DNA. Major Histocompatibility Complex (MHC) identity of each NT-hESC with the patient's showed immunological compatibility, important for eventual transplantation. With the generation of these NT-hESCs, evaluations of genetic and epigenetic stability can be made. Additional work remains regarding the development of reliable directed differentiation and the elimination of remaining animal components. Prior to clinical use of these cells, preclinical evidence is required to prove that transplantation of differentiated-NT-hESCs can be safe, effective and tolerated.


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