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Published Online September 30, 2004
Science DOI: 10.1126/science.1103185

Reports

Submitted on July 23, 2004
Accepted on September 16, 2004

Accumulation of Mn(II) in Deinococcus radiodurans Facilitates Gamma-Radiation Resistance

M. J. Daly 1*, E. K. Gaidamakova 1, V. Y. Matrosova 1, A. Vasilenko 1, M. Zhai 1, A. Venkateswaran 1, M. Hess 1, M. V. Omelchenko 2, H. M. Kostandarithes 3, K. S. Makarova 4, L. P. Wackett 5, J. K. Fredrickson 3, D. Ghosal 1

1 Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA.
2 Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA; National Institutes of Health, Bethesda, MD 20894, USA.
3 Pacific Northwest National Laboratory, Richland, WA 99352, USA.
4 National Institutes of Health, Bethesda, MD 20894, USA.
5 University of Minnesota, St. Paul, MN 55108, USA.

* To whom correspondence should be addressed.
M. J. Daly , E-mail: mdaly{at}usuhs.mil

Deinococcus radiodurans is extremely resistant to ionizing radiation. How this bacterium can grow under chronic {gamma}radiation (50 Gy/hour) or recover from acute doses greater than 10 kGy is unknown. We show that D. radiodurans accumulates very high intracellular manganese and low iron levels compared to radiation sensitive bacteria, and resistance exhibits a concentration-dependent response to Mn(II). Among the most radiation-resistant bacterial groups reported, Deinococcus, Enterococcus, Lactobacillus, and cyanobacteria accumulate Mn(II). In contrast, Shewanella oneidensis and Pseudomonas putida have high Fe but low intracellular Mn concentrations and are very sensitive. We propose that Mn(II) accumulation facilitates recovery from radiation injury.


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