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Published Online February 12, 2004
Science DOI: 10.1126/science.1094515

Reports

Submitted on December 9, 2003
Accepted on February 4, 2004

Evidence of a Pluripotent Human Embryonic Stem Cell Line Derived from a Cloned Blastocyst

Woo Suk Hwang 1*, Young June Ryu 2, Jong Hyuk Park 3, Eul Soon Park 2, Eu Gene Lee 2, Ja Min Koo 4, Hyun Yong Chun 2, Byeong Chun Lee 2, Sung Keun Kang 2, Sun Jong Kim 3, Curie Ahn 5, Jung Hye Hwang 6, Ky Young Park 7, Jose B. Cibelli 8, Shin Yong Moon 5*

1 College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea; School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea.
2 College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
3 Medical Research Center, Mizmedi Hospital, Seoul, 135-280, Korea.
4 Gachon Medical School, Incheon, 417-840, Korea.
5 College of Medicine, Seoul National University, Seoul, 110-744, Korea.
6 School of Medicine, Hanyang University, Seoul, 471-701, Korea.
7 College of Natural Science, Sunchon National University, Sunchon, 540-742, Korea.
8 Department of Animal Science-Physiology, Michigan State University, East Lansing, MI 48824, USA.

* To whom correspondence should be addressed. E-mail: hwangws{at}snu.ac.kr, shmoon{at}plaza.snu.ac.kr.

Somatic cell nuclear transfer (SCNT) technology has recently been used to generate animals with a common genetic composition. In this study, we report the derivation of a pluripotent embryonic stem cell line (SCNT-hES-1) from a cloned human blastocyst. SCNT-hES-1 cells display typical ES cell morphology and cell surface markers and are capable of differentiating into embryoid bodies in vitro and of forming teratomas in vivo containing cell derivatives from all three embryonic germ layers in SCID mice. After continuous proliferation for >70 passages, SCNT-hES-1 cells maintain normal karyotypes and are genetically identical to the somatic nuclear donor cells. Although we cannot completely exclude the possibility of a parthenogenetic origin of the cells, imprinting analyses provide support that the derived human ES cells have a somatic cell nuclear transfer origin.


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