Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Originally published in Science Express on 2 January 2004
Science 23 January 2004:
Vol. 303. no. 5657, pp. 540 - 543
DOI: 10.1126/science.1091403
Prev | Table of Contents | Next

Reports

A Map of the Interactome Network of the Metazoan C. elegans

Siming Li,1* Christopher M. Armstrong,1* Nicolas Bertin,1* Hui Ge,1* Stuart Milstein,1* Mike Boxem,1* Pierre-Olivier Vidalain,1* Jing-Dong J. Han,1* Alban Chesneau,1,2* Tong Hao,1 Debra S. Goldberg,3 Ning Li,1 Monica Martinez,1 Jean-François Rual,1,4 Philippe Lamesch,1,4 Lai Xu,5{dagger} Muneesh Tewari,1 Sharyl L. Wong,3 Lan V. Zhang,3 Gabriel F. Berriz,3 Laurent Jacotot,1{ddagger} Philippe Vaglio,1{ddagger} Jérôme Reboul,1§ Tomoko Hirozane-Kishikawa,1 Qianru Li,1 Harrison W. Gabel,1 Ahmed Elewa,1|| Bridget Baumgartner,5 Debra J. Rose,6 Haiyuan Yu,7 Stephanie Bosak,8 Reynaldo Sequerra,8 Andrew Fraser,9 Susan E. Mango,10 William M. Saxton,6 Susan Strome,6 Sander van den Heuvel,11 Fabio Piano,12 Jean Vandenhaute,4 Claude Sardet,2 Mark Gerstein,7 Lynn Doucette-Stamm,8 Kristin C. Gunsalus,12 J. Wade Harper,5{dagger} Michael E. Cusick,1 Frederick P. Roth,3 David E. Hill,1 Marc Vidal1#

To initiate studies on how protein-protein interaction (or "interactome") networks relate to multicellular functions, we have mapped a large fraction of the Caenorhabditis elegans interactome network. Starting with a subset of metazoan-specific proteins, more than 4000 interactions were identified from high-throughput, yeast two-hybrid (HT=Y2H) screens. Independent coaffinity purification assays experimentally validated the overall quality of this Y2H data set. Together with already described Y2H interactions and interologs predicted in silico, the current version of the Worm Interactome (WI5) map contains ~5500 interactions. Topological and biological features of this interactome network, as well as its integration with phenome and transcriptome data sets, lead to numerous biological hypotheses.

1 Dana-Farber Cancer Institute and Department of Genetics, Harvard Medical School, 44 Binney Street, Boston, MA 02115, USA.
2 Institut de Génétique Moléculaire, Centre National de la Recherche Scientifique UMR 5535, 1919 Route de Mende, 34293 Montpellier Cedex 5, France.
3 Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 250 Longwood Avenue, Boston, MA 02115, USA.
4 Unité de Recherche en Biologie Moléculaire, Facultés Notre-Dame de la Paix, 61 Rue de Bruxelles, 5000 Namur, Belgium.
5 Verna and Marrs Department of Biochemistry and Molecular Biology, Program in Cell and Molecular Biology, Biophysics, and Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA.
6 Department of Biology, Indiana University, Jordan Hall 142, 1001 East Third Street, Bloomington, IN 47405, USA.
7 Department of Molecular Biophysics and Biochemistry and Department of Computer Science, Yale University, 266 Whitney Avenue, New Haven, CT 06520, USA.
8 Agencourt Bioscience Corporation, 100 Cummings Center, Suite 107G, Beverly, MA 01915, USA.
9 The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK.
10 Huntsman Cancer Institute, University of Utah, 2000 Circle of Hope, Salt Lake City, UT 84112, USA.
11 Massachusetts General Hospital Cancer Center, Building 149, 13th Street, Charlestown, MA 02129, USA.
12 Department of Biology, New York University, 1009 Silver Building, 100 Washington Square East, New York, NY 10003, USA.



* These authors contributed equally to this work.

{dagger} Present address: Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA.

{ddagger} Present address: Modul-Bio, 232 Boulevard Sainte-Marguerite, 13009 Marseille, France.

§ Present address: INSERM, Unité 119, Institut Paoli Calmettes, 13009 Marseille, France.

|| Present address: Program in Gene Function and Expression, University of Massachusetts, 55 Lake Avenue, North Worcester, MA 01605, USA.

Present address: Center for Cancer Systems Biology and Department of Cancer Biology, Dana-Farber Cancer Institute and Department of Genetics, Harvard Medical School, 44 Binney Street, Boston, MA 02115, USA.

# To whom correspondence should be addressed. E-mail: marc_vidal{at}dfci.harvard.edu.

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
A complex-centric view of protein network evolution.
N. Yosef, M. Kupiec, E. Ruppin, and R. Sharan (2009)
Nucleic Acids Res. 37, e88
   Abstract »    Full Text »    PDF »
SNOW, a web-based tool for the statistical analysis of protein-protein interaction networks.
P. Minguez, S. Gotz, D. Montaner, F. Al-Shahrour, and J. Dopazo (2009)
Nucleic Acids Res. 37, W109-W114
   Abstract »    Full Text »    PDF »
Global networks of functional coupling in eukaryotes from comprehensive data integration.
A. Alexeyenko and E. L.L. Sonnhammer (2009)
Genome Res. 19, 1107-1116
   Abstract »    Full Text »    PDF »
Deciphering Signaling Outcomes from a System of Complex Networks.
R. C. Hsueh, M. Natarajan, I. Fraser, B. Pond, J. Liu, S. Mumby, H. Han, L. I. Jiang, M. I. Simon, R. Taussig, et al. (2009)
Science Signaling 2, ra22
   Abstract »    Full Text »    PDF »
Progress and challenges in predicting protein-protein interaction sites.
I. Ezkurdia, L. Bartoli, P. Fariselli, R. Casadio, A. Valencia, and M. L. Tress (2009)
Brief Bioinform 10, 233-246
   Abstract »    Full Text »    PDF »
The ALP-Enigma Protein ALP-1 Functions in Actin Filament Organization to Promote Muscle Structural Integrity in Caenorhabditis elegans.
H.-F. Han and M. C. Beckerle (2009)
Mol. Biol. Cell 20, 2361-2370
   Abstract »    Full Text »    PDF »
Control of Rapsyn Stability by the CUL-3-containing E3 Ligase Complex.
S. Nam, K. Min, H. Hwang, H.-o. Lee, J. H. Lee, J. Yoon, H. Lee, S. Park, and J. Lee (2009)
J. Biol. Chem. 284, 8195-8206
   Abstract »    Full Text »    PDF »
Gene divergence and pathway duplication in the metabolic network of yeast and digital organisms.
P. Gerlee, T. Lundh, B. Zhang, and A.R.A. Anderson (2009)
J R Soc Interface
   Abstract »    Full Text »    PDF »
RACK-1 Directs Dynactin-dependent RAB-11 Endosomal Recycling during Mitosis in Caenorhabditis elegans.
E. Ai, D. S. Poole, and A. R. Skop (2009)
Mol. Biol. Cell 20, 1629-1638
   Abstract »    Full Text »    PDF »
The Cartographers toolbox: building bigger and better human protein interaction networks.
C. M. Sanderson (2009)
Brief Funct Genomic Proteomic
   Abstract »    Full Text »    PDF »
Versatile selection technology for intracellular protein-protein interactions mediated by a unique bacterial hitchhiker transport mechanism.
D. Waraho and M. P. DeLisa (2009)
PNAS 106, 3692-3697
   Abstract »    Full Text »    PDF »
Functional interactions between the ciliopathy-associated Meckel syndrome 1 (MKS1) protein and two novel MKS1-related (MKSR) proteins.
N. J. Bialas, P. N. Inglis, C. Li, J. F. Robinson, J. D. K. Parker, M. P. Healey, E. E. Davis, C. D. Inglis, T. Toivonen, D. C. Cottell, et al. (2009)
J. Cell Sci. 122, 611-624
   Abstract »    Full Text »    PDF »
The Role of Protein Phosphatase 4 in Regulating Microtubule Severing in the Caenorhabditis elegans Embryo.
X. Han, J.-E. Gomes, C. L. Birmingham, L. Pintard, A. Sugimoto, and P. E. Mains (2009)
Genetics 181, 933-943
   Abstract »    Full Text »    PDF »
A Rice Kinase-Protein Interaction Map.
X. Ding, T. Richter, M. Chen, H. Fujii, Y. S. Seo, M. Xie, X. Zheng, S. Kanrar, R. A. Stevenson, C. Dardick, et al. (2009)
Plant Physiology 149, 1478-1492
   Abstract »    Full Text »    PDF »
Precision and recall estimates for two-hybrid screens.
H. Huang and J. S. Bader (2009)
Bioinformatics 25, 372-378
   Abstract »    Full Text »    PDF »
Experimental advances in the characterization of metazoan gene regulatory networks.
B. Deplancke (2009)
Brief Funct Genomic Proteomic 8, 12-27
   Abstract »    Full Text »    PDF »
Challenges and Rewards of Interaction Proteomics.
S. J. Wodak, S. Pu, J. Vlasblom, and B. Seraphin (2009)
Mol. Cell. Proteomics 8, 3-18
   Abstract »    Full Text »    PDF »
ConsensusPathDB--a database for integrating human functional interaction networks.
A. Kamburov, C. Wierling, H. Lehrach, and R. Herwig (2009)
Nucleic Acids Res. 37, D623-D628
   Abstract »    Full Text »    PDF »
PIPs: human protein-protein interaction prediction database.
M. D. McDowall, M. S. Scott, and G. J. Barton (2009)
Nucleic Acids Res. 37, D651-D656
   Abstract »    Full Text »    PDF »
Role of the Caenorhabditis elegans Shc Adaptor Protein in the c-Jun N-Terminal Kinase Signaling Pathway.
T. Mizuno, K. Fujiki, A. Sasakawa, N. Hisamoto, and K. Matsumoto (2008)
Mol. Cell. Biol. 28, 7041-7049
   Abstract »    Full Text »    PDF »
The Transcriptional Network Controlling Pluripotency in ES Cells.
S.H. Orkin, J. Wang, J. Kim, J. Chu, S. Rao, T.W. Theunissen, X. Shen, and D.N. Levasseur (2008)
Cold Spring Harb Symp Quant Biol
   Abstract »    PDF »
Caenorhabditis elegans: An Emerging Model in Biomedical and Environmental Toxicology.
M. C. K. Leung, P. L. Williams, A. Benedetto, C. Au, K. J. Helmcke, M. Aschner, and J. N. Meyer (2008)
Toxicol. Sci. 106, 5-28
   Abstract »    Full Text »    PDF »
Protein networks markedly improve prediction of subcellular localization in multiple eukaryotic species.
K. Lee, H.-Y. Chuang, A. Beyer, M.-K. Sung, W.-K. Huh, B. Lee, and T. Ideker (2008)
Nucleic Acids Res. 36, e136
   Abstract »    Full Text »    PDF »
Molecular Characterization of Organelle-Type Nudix Hydrolases in Arabidopsis.
T. Ogawa, K. Yoshimura, H. Miyake, K. Ishikawa, D. Ito, N. Tanabe, and S. Shigeoka (2008)
Plant Physiology 148, 1412-1424
   Abstract »    Full Text »    PDF »
Genes involved in pre-mRNA 3'-end formation and transcription termination revealed by a lin-15 operon Muv suppressor screen.
M. Cui, M. A. Allen, A. Larsen, M. MacMorris, M. Han, and T. Blumenthal (2008)
PNAS 105, 16665-16670
   Abstract »    Full Text »    PDF »
High-Quality Binary Protein Interaction Map of the Yeast Interactome Network.
H. Yu, P. Braun, M. A. Yildirim, I. Lemmens, K. Venkatesan, J. Sahalie, T. Hirozane-Kishikawa, F. Gebreab, N. Li, N. Simonis, et al. (2008)
Science 322, 104-110
   Abstract »    Full Text »    PDF »
SHC-1/p52Shc targets the insulin/IGF-1 and JNK signaling pathways to modulate life span and stress response in C. elegans.
E. Neumann-Haefelin, W. Qi, E. Finkbeiner, G. Walz, R. Baumeister, and M. Hertweck (2008)
Genes & Dev. 22, 2721-2735
   Abstract »    Full Text »    PDF »
ADBP-1 Regulates an ADAR RNA-Editing Enzyme to Antagonize RNA-Interference-Mediated Gene Silencing in Caenorhabditis elegans.
H. Ohta, M. Fujiwara, Y. Ohshima, and T. Ishihara (2008)
Genetics 180, 785-796
   Abstract »    Full Text »    PDF »
Message-passing algorithms for the prediction of protein domain interactions from protein-protein interaction data.
M. Iqbal, A. A. Freitas, C. G. Johnson, and M. Vergassola (2008)
Bioinformatics 24, 2064-2070
   Abstract »    Full Text »    PDF »
Natural Selection against Protein Aggregation on Self-Interacting and Essential Proteins in Yeast, Fly, and Worm.
Y. Chen and N. V. Dokholyan (2008)
Mol. Biol. Evol. 25, 1530-1533
   Abstract »    Full Text »    PDF »
The Croonian lecture 2006 Structure of the living cell.
I. D Campbell (2008)
Phil Trans R Soc B 363, 2379-2391
   Abstract »    Full Text »    PDF »
Genetic networks for the functional study of genomes.
A. G. Pisabarro, G. Perez, J. L. Lavin, and L. Ramirez (2008)
Brief Funct Genomic Proteomic 7, 249-263
   Abstract »    Full Text »    PDF »
A global pathway crosstalk network.
Y. Li, P. Agarwal, and D. Rajagopalan (2008)
Bioinformatics 24, 1442-1447
   Abstract »    Full Text »    PDF »
PRINCESS, a Protein Interaction Confidence Evaluation System with Multiple Data Sources.
D. Li, W. Liu, Z. Liu, J. Wang, Q. Liu, Y. Zhu, and F. He (2008)
Mol. Cell. Proteomics 7, 1043-1052
   Abstract »    Full Text »    PDF »
Identification of innate immunity genes and pathways using a comparative genomics approach.
S. Alper, R. Laws, B. Lackford, W. A. Boyd, P. Dunlap, J. H. Freedman, and D. A. Schwartz (2008)
PNAS 105, 7016-7021
   Abstract »    Full Text »    PDF »
From the Cover: Estimating the size of the human interactome.
M. P. H. Stumpf, T. Thorne, E. de Silva, R. Stewart, H. J. An, M. Lappe, and C. Wiuf (2008)
PNAS 105, 6959-6964
   Abstract »    Full Text »    PDF »
AMP-activated Protein Kinase and p38 MAPK Activate O-GlcNAcylation of Neuronal Proteins during Glucose Deprivation.
W. D. Cheung and G. W. Hart (2008)
J. Biol. Chem. 283, 13009-13020
   Abstract »    Full Text »    PDF »
Studying gene function in Caenorhabditis elegans using RNA-mediated interference.
E. M. Maine (2008)
Brief Funct Genomic Proteomic 7, 184-194
   Abstract »    Full Text »    PDF »
Network-guided genetic screening: building, testing and using gene networks to predict gene function.
B. Lehner and I. Lee (2008)
Brief Funct Genomic Proteomic 7, 217-227
   Abstract »    Full Text »    PDF »
Functional Redundancy of the B9 Proteins and Nephrocystins in Caenorhabditis elegans Ciliogenesis.
C. L. Williams, M. E. Winkelbauer, J. C. Schafer, E. J. Michaud, and B. K. Yoder (2008)
Mol. Biol. Cell 19, 2154-2168
   Abstract »    Full Text »    PDF »
Fitting a geometric graph to a protein-protein interaction network.
D. J. Higham, M. Rasajski, and N. Przulj (2008)
Bioinformatics 24, 1093-1099
   Abstract »    Full Text »    PDF »
From individual Wnt pathways towards a Wnt signalling network.
H. A Kestler and M. Kuhl (2008)
Phil Trans R Soc B 363, 1333-1347
   Abstract »    Full Text »    PDF »
A new way to explore the world of extracellular protein interactions.
C. M. Sanderson (2008)
Genome Res. 18, 517-520
   Abstract »    Full Text »    PDF »
An optimized split-ubiquitin cDNA-library screening system to identify novel interactors of the human Frizzled 1 receptor.
D. Dirnberger, M. Messerschmid, and R. Baumeister (2008)
Nucleic Acids Res. 36, e37
   Abstract »    Full Text »    PDF »
Genome-wide inference of protein interaction sites: lessons from the yeast high-quality negative protein-protein interaction dataset.
J. Guo, X. Wu, D.-Y. Zhang, and K. Lin (2008)
Nucleic Acids Res. 36, 2002-2011
   Abstract »    Full Text »    PDF »
New Connections across Pathways and Cellular Processes: Industrialized Mutant Screening Reveals Novel Associations between Diverse Phenotypes in Arabidopsis.
Y. Lu, L. J. Savage, I. Ajjawi, K. M. Imre, D. W. Yoder, C. Benning, D. DellaPenna, J. B. Ohlrogge, K. W. Osteryoung, A. P. Weber, et al. (2008)
Plant Physiology 146, 1482-1500
   Abstract »    Full Text »    PDF »
An assessment of the uses of homologous interactions.
R. Saeed and C. Deane (2008)
Bioinformatics 24, 689-695
   Abstract »    Full Text »    PDF »
Oxidative stress in Caenorhabditis elegans: protective effects of the Omega class glutathione transferase (GSTO-1).
C. Burmeister, K. Luersen, A. Heinick, A. Hussein, M. Domagalski, R. D. Walter, and E. Liebau (2008)
FASEB J 22, 343-354
   Abstract »    Full Text »    PDF »
Firefly Luciferase Complementation Imaging Assay for Protein-Protein Interactions in Plants.
H. Chen, Y. Zou, Y. Shang, H. Lin, Y. Wang, R. Cai, X. Tang, and J.-M. Zhou (2008)
Plant Physiology 146, 368-376
   Abstract »    Full Text »    PDF »
Improving yeast two-hybrid screening systems.
M. Koegl and P. Uetz (2008)
Brief Funct Genomic Proteomic
   Abstract »    Full Text »    PDF »
Hypothesis-based RNAi screening identifies neuroprotective genes in a Parkinson's disease model.
S. Hamamichi, R. N. Rivas, A. L. Knight, S. Cao, K. A. Caldwell, and G. A. Caldwell (2008)
PNAS 105, 728-733
   Abstract »    Full Text »    PDF »
DOMINE: a database of protein domain interactions.
B. Raghavachari, A. Tasneem, T. M. Przytycka, and R. Jothi (2008)
Nucleic Acids Res. 36, D656-D661
   Abstract »    Full Text »    PDF »
AtPID: Arabidopsis thaliana protein interactome database an integrative platform for plant systems biology.
J. Cui, P. Li, G. Li, F. Xu, C. Zhao, Y. Li, Z. Yang, G. Wang, Q. Yu, Y. Li, et al. (2008)
Nucleic Acids Res. 36, D999-D1008
   Abstract »    Full Text »    PDF »
Yeast two-hybrid interaction partner screening through in vivo Cre-mediated Binary Interaction Tag generation.
A. R. Hastie and S. C. Pruitt (2007)
Nucleic Acids Res. 35, e141
   Abstract »    Full Text »    PDF »
Examining protein protein interactions using endogenously tagged yeast arrays: The Cross-and-Capture system.
B. Suter, M. J. Fetchko, R. Imhof, C. I. Graham, I. Stoffel-Studer, C. Zbinden, M. Raghavan, L. Lopez, L. Beneti, J. Hort, et al. (2007)
Genome Res. 17, 1774-1782
   Abstract »    Full Text »    PDF »
A Novel Cysteine Cross-linking Method Reveals a Direct Association between Claudin-1 and Tetraspanin CD9.
O. V. Kovalenko, X. H. Yang, and M. E. Hemler (2007)
Mol. Cell. Proteomics 6, 1855-1867
   Abstract »    Full Text »    PDF »
A Predicted Interactome for Arabidopsis.
J. Geisler-Lee, N. O'Toole, R. Ammar, N. J. Provart, A. H. Millar, and M. Geisler (2007)
Plant Physiology 145, 317-329
   Abstract »    Full Text »    PDF »
Draft Genome of the Filarial Nematode Parasite Brugia malayi.
E. Ghedin, S. Wang, D. Spiro, E. Caler, Q. Zhao, J. Crabtree, J. E. Allen, A. L. Delcher, D. B. Guiliano, D. Miranda-Saavedra, et al. (2007)
Science 317, 1756-1760
   Abstract »    Full Text »    PDF »
APID2NET: unified interactome graphic analyzer.
J. Hernandez-Toro, C. Prieto, and J. De Las Rivas (2007)
Bioinformatics 23, 2495-2497
   Abstract »    Full Text »    PDF »
Automated data integration for developmental biological research.
W. Zhong and P. W. Sternberg (2007)
Development 134, 3227-3238
   Abstract »    Full Text »    PDF »
GLH-1, the C. elegans P granule protein, is controlled by the JNK KGB-1 and by the COP9 subunit CSN-5.
A. M. Orsborn, W. Li, T. J. McEwen, T. Mizuno, E. Kuzmin, K. Matsumoto, and K. L. Bennett (2007)
Development 134, 3383-3392
   Abstract »    Full Text »    PDF »
MyD88-5 links mitochondria, microtubules, and JNK3 in neurons and regulates neuronal survival.
Y. Kim, P. Zhou, L. Qian, J.-Z. Chuang, J. Lee, C. Li, C. Iadecola, C. Nathan, and A. Ding (2007)
J. Exp. Med. 204, 2063-2074
   Abstract »    Full Text »    PDF »
MicroRNA regulation of human protein protein interaction network.
H. Liang and W.-H. Li (2007)
RNA 13, 1402-1408
   Abstract »    Full Text »    PDF »
How old genes make a new head: redeployment of Six and Eya genes during the evolution of vertebrate cranial placodes.
G. Schlosser (2007)
Integr. Comp. Biol. 47, 343-359
   Abstract »    Full Text »    PDF »
A multidimensional analysis of genes mutated in breast and colorectal cancers.
J. Lin, C. M. Gan, X. Zhang, S. Jones, T. Sjoblom, L. D. Wood, D. W. Parsons, N. Papadopoulos, K. W. Kinzler, B. Vogelstein, et al. (2007)
Genome Res. 17, 1304-1318
   Abstract »    Full Text »    PDF »
Structural templates predict novel protein interactions and targets from pancreas tumour gene expression data.
G. Dawelbait, C. Winter, Y. Zhang, C. Pilarsky, R. Grutzmann, J.-C. Heinrich, and M. Schroeder (2007)
Bioinformatics 23, i115-i124
   Abstract »    Full Text »    PDF »
Computational prediction of host-pathogen protein protein interactions.
M. D. Dyer, T. M. Murali, and B. W. Sobral (2007)
Bioinformatics 23, i159-i166
   Abstract »    Full Text »    PDF »
CIF-1, a Shared Subunit of the COP9/Signalosome and Eukaryotic Initiation Factor 3 Complexes, Regulates MEL-26 Levels in the Caenorhabditis elegans Embryo.
S. Luke-Glaser, M. Roy, B. Larsen, T. Le Bihan, P. Metalnikov, M. Tyers, M. Peter, and L. Pintard (2007)
Mol. Cell. Biol. 27, 4526-4540
   Abstract »    Full Text »    PDF »
Variable gene expression in eukaryotes: a network perspective.
P. J. Wittkopp (2007)
J. Exp. Biol. 210, 1567-1575
   Abstract »    Full Text »    PDF »
Getting connected: analysis and principles of biological networks.
X. Zhu, M. Gerstein, and M. Snyder (2007)
Genes & Dev. 21, 1010-1024
   Abstract »    Full Text »    PDF »
The Biologically Relevant Targets and Binding Affinity Requirements for the Function of the Yeast Actin-Binding Protein 1 Src-Homology 3 Domain Vary With Genetic Context.
J. Haynes, B. Garcia, E. J. Stollar, A. Rath, B. J. Andrews, and A. R. Davidson (2007)
Genetics 176, 193-208
   Abstract »    Full Text »    PDF »
Sensory Ciliogenesis in Caenorhabditis elegans: Assignment of IFT Components into Distinct Modules Based on Transport and Phenotypic Profiles.
G. Ou, M. Koga, O. E. Blacque, T. Murayama, Y. Ohshima, J. C. Schafer, C. Li, B. K. Yoder, M. R. Leroux, and J. M. Scholey (2007)
Mol. Biol. Cell 18, 1554-1569
   Abstract »    Full Text »    PDF »
Inferring genome-wide functional linkages in E. coli by combining improved genome context methods: Comparison with high-throughput experimental data.
S. Yellaboina, K. Goyal, and S. C. Mande (2007)
Genome Res. 17, 527-535
   Abstract »    Full Text »    PDF »
Functional genomics and biochemical characterization of the C. elegans orthologue of the Machado-Joseph disease protein ataxin-3.
A.-J. Rodrigues, G. Coppola, C. Santos, M. d. C. Costa, M. Ailion, J. Sequeiros, D. H. Geschwind, and P. Maciel (2007)
FASEB J 21, 1126-1136
   Abstract »    Full Text »    PDF »
A Novel Approach for Generating Full-length, High Coverage Allele Libraries for the Analysis of Protein Interactions.
P. N. Gray, K. J. Busser, and T. G. Chappell (2007)
Mol. Cell. Proteomics 6, 514-526
   Abstract »    Full Text »    PDF »
Comparison of human protein protein interaction maps.
M. E. Futschik, G. Chaurasia, and H. Herzel (2007)
Bioinformatics 23, 605-611
   Abstract »    Full Text »    PDF »
Gateway-Based Destination Vectors for Functional Analyses of Bacterial ORFeomes: Application to the Min System in Brucella abortus.
R. Hallez, J.-J. Letesson, J. Vandenhaute, and X. De Bolle (2007)
Appl. Envir. Microbiol. 73, 1375-1379
   Abstract »    Full Text »    PDF »
MRG-1, an autosome-associated protein, silences X-linked genes and protects germline immortality in Caenorhabditis elegans.
T. Takasaki, Z. Liu, Y. Habara, K. Nishiwaki, J.-i. Nakayama, K. Inoue, H. Sakamoto, and S. Strome (2007)
Development 134, 757-767
   Abstract »    Full Text »    PDF »
Two-dimensional Blue Native/SDS Gel Electrophoresis of Multiprotein Complexes from Helicobacter pylori.
S. Pyndiah, J. P. Lasserre, A. Menard, S. Claverol, V. Prouzet-Mauleon, F. Megraud, F. Zerbib, and M. Bonneu (2007)
Mol. Cell. Proteomics 6, 193-206
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)